The Cinderella story of "Fast" Eddie Chambers' latest trip to Germany ended badly for the American heavyweight contender tonight at the Esprit Arena in Dusseldorf.
The 27 year old Philadelphia based stylist was he latest challenger to prove unable to solve the problems posed by Heavyweight Champion Wladimir Klitschko, a giant of a man possessing tremendous boxing skill and power.
The bout started with Chambers finding the target with the jab and right hand but soon developed into a one-sided undertaking featuring the best jab in the division as Klitschko controlled the pace of the bout and kept the challenger at a distance.
The middle rounds weren't particularly eventful but Klitschko began to mix in his accurate and heavy right hand, wearing Chambers down in the process.
By the ninth, Klitschko added snapping left hooks to the mix as Chambers began to map out survival strategies in the face of such an imposing antagonist.
In the 12th and final frame, Klitschko poured it on and knocked out the American with a perfect left hook with just five seconds left to go in the fight.
Wladimir Klitschko improves to 54-3 (38) while retaining the IBF, WBO and IBO crowns. Eddie Chambers drops to 35-2 (18) and sees a five bout win streak snapped.
There were also fireworks on the undercard as former cruiserweight contender Johnathon Banks, 24-1 (17), ended the hopes of Travis Walker, 34-4-1 (28), in the sixth to annex the vacant NABF heavyweight title.
Unbeaten heavyweight prospect Alexander Ustinov, 20-0 (15), stayed perfect as Ed Mahone, 24-10-2 (23), didn't come out for the fourth.
Ex-British and European Union heavyweight champ Michael Sprott, 32-14 (17), KO'd Werner Kreiskott, 7-14-1 (5), in the first and is due to face UK rival Audley Harrison for the vacant European title in April in a rematch.